Animal & plant health laboratory Saône-et-Loire Agrivalys 71
  • Phone +33 (0)3 85 33 52 20
  • Opening hours Monday to Friday, 8am-Noon / 1:30-5pm (4:30pm on Fridays)
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Departmental laboratory for plant health analyzes of vines
Departmental laboratory for plant health analyzes of vines
Departmental laboratory for plant health analyzes of vines
Departmental laboratory for plant health analyzes of vines

Testing for vine viruses, Golden Flavescence, Xylella fastidiosa

Agrivalys 71 is an analysis laboratory in Burgundy specialising in plant health and diseases of the vine : Golden Flavescence, Pierce’s Disease, Grapevine Leafroll Disease and Grapevine Fanleaf Virus etc.

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Monitoring, researching, improving

Testing vines

Our laboratory tests samples from the vine and detects pathogen agents that cause symptomatic or asymptomatic diseases.

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Methodology Our methods
ELISA

Detecting viral antigens from vine samples (wood, roots or leaves).

PCR

DNA polymerase amplification (specific region of a nucleic acid) to detect it and study it.

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List of tests by pathogen agent

Tests for vines by pathology

Our laboratory diagnoses vine diseases caused by Golden Flavescence, Black Wood, Xylella fastidiosa and vine viruses (Grapevine Leafroll and Grapevine Fanleaf).

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Description :

Golden Flavescence or Flavescence Dorée (FD) and Black Wood (BW) are two diseases belonging to the category of grapevine yellows in Europe. These two diseases have similar symptoms and yet they are linked to two different phytoplasma belonging respectively to the Elm Yellows (16 SrV) and the Stolbur group (16 SrXll).

Golden Flavescence is a regulated vineyard disease present in France and transmitted through an insect vector (a leafhopper).

Symptoms :
  • Leafroll
  • Change in colour (turning red or yellow) evolving towards leaf necrosis
  • Wilting of grape bunches
  • Non-hardening of branches
Method : PCR
Matrix : Leaves with petiols
Description :

Xylella fastidiosa is a xylem bacteria, mainly transmitted by insect vectors, that can infect more than 500 species of plant. It is a regulated disease and there have been outbreaks in France since 2015. When is affects the vine it is known as Pierce’s Disease.

Symptoms :
  • Leaf burns
  • Generalised shrivelling of the plant
  • Foliar chlorosis
  • Lack of lignification (hardening)
  • Leaves turning yellow or red
Testing must be carried out in the event of any suspicion of disease. However, the disease can often be asymptomatic.
Method : PCR
Matrix : Leaves
Leafroll Disease (GLRaV-1, GLRaV-2 and GLRaV-3) Description :

Grapevine Leafroll is associated with viruses that all belong to the Closteroviridae group, three species of which are found in France: GLRaV-1, GLRaV-2 and GLRaV-3.

During infection, obstruction of the phloem vessels (cochineal) occurs. Dissemination is through contaminated plant material or insect vectors.

Symptoms (more evident with GLRaV-1 and GLRaV-3) :
  • Rolling of the blade of the leaf.
  • In black grape varieties, reddening of the leaf spreads throughout the blade.
  • In white grape varieties the leaves turn yellow.

Fanleaf Disease (ArMV and GFLV)

Description :

Fanleaf is a viral disease associated with two nepoviruses in France : Grapevine Fanleaf Virus (GFLV) is the most common, and the rarer Arabis Mosaic Virus (ArMV).

The symptoms caused by these viruses are identical but are transmitted by two different vector nematodes (Xiphinema).

Symptoms :
  • On branches : shortening of internodes, abnormal growth.
  • Deformation and yellowing that may be localised or spread to the whole leaf.
  • On leaves : deformations, vein anomalies.
  • Reduction in yield and quality.
Method : ELISA
Matrix : Wood, leaves, roots